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Summaries of Research Conducted with LeafSourceTM

Conducted at Mercer University Southern School of Pharmacy

Atlanta, Georgia

April 2003

Evaluate the Effects of

LeafSourceTM

on Lymphocyte Responsiveness

Purpose

This experiment was carried out to determine if LeafSourceTM has any beneficial effect on lymphocyte responsiveness.

Results and Discussion

The immune system has been divided into two major divisions: the cellular and the humoral immune systems. The common link between both of these systems is the

T-lymphocyte. The activation of this cell by most specific antigens is absolutely essential for either of these two immune systems to function properly. In this laboratory study we use non-specific antigens (concanavalin A Con A) to activate T-lymphocytes. By using the Con A we can evaluate the responsiveness of the T-lymphocytes which will give us an idea of the function of the cellular and humoral immunity. The T cell and the cellular immune system are involved in both cellular and humoral immunity. The responsiveness of T-lymphocytes to Con A accurately reflects the state of the immune system. Therefore, using Con A and observing its ability and affect on lymphocytes in the presence of LeafSourceTM will give us valuable information on the ability of this product to affect lymphocyte responsiveness.

The experiment that we conducted evaluated the effects of LeafSourceTM on normal rat spleen lymphocyte responsiveness to Con A. LeafSourceTM was incorporated into the food pellets and placed in food containers where they were available to the rats at all times. Liquid concentrate LeafSourceTM was incorporated into the drinking water. There was no other source of water available to the rats. The animals were exposed to the two dosage regimes for 30 days. At the end of that time period, animals were anesthetized followed by immediate removal of their spleens and the lymphocytes isolated. These cells were cultured for 72 hours and their responsiveness to 2ug/ml of Con A was determined.


The lymphocytes from the group of rats receiving 0.5% of LeafSourceTM showed almost a 7% increase above controls. The lymphocytes isolate from the two dosages of liquid concentrate LeafSourceTM showed 7% responsiveness to Con A at the lower rate, and nearly a 14% responsiveness to Con A at the higher rate.

Therefore, there appears to be a trend towards increased lymphocyte responsiveness to Con A in both the treatments with LeafSourceTM. The importance of these results is to be determined. Increases in lymphocyte responsiveness suggests a greater ability of the cellular, and also the humoral, immune systems to respond to foreign substances including microorganisms and other unknown antigens. This could lead to reduced systemic infections, increased removal of foreign antigens or antigens found within the body that are growing abnormally or other unknown benefits.

The data that we have accumulated thus far and from other sources suggest that LeafSourceTM may have the ability to increase the response of the immune system. Based on our results, the possible mechanisms associated with this increase could be either an increase in lymphocyte responsiveness and/or an increase in lymphocyte numbers. Taken together, these results suggest that chronic dosing with LeafSourceTM may have an effect on the immune system that could be beneficial in a number of different disease states.


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